COX2/PTGS2 Antibody

• Catalog Number: orb1294345
• Category: Antibodies
• Description: Rabbit polyclonal antibody to COX2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:500 - 1:1000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:200
• Conjugation: Unconjugated
• Target: COX2 / PTGS2
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-COX2 antibody - at 1/1000 dilution, Lysates at 35 μg per lane. This blot was produced using a 7.5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294345 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling COX2 with orb1294345 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling COX2 with orb1294345 at 1/100.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294345 anti-COX2 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling COX2 with orb1294345 at 1/100.