COVID-19 & SARS-CoV S glycoprotein Antibody [CR3022], Human IgG1

• Catalog Number: orb1671578
• Category: Antibodies
• Description: COVID-19 & SARS-CoV S glycoprotein Antibody [CR3022], Human IgG1
• Clonality: Recombinant
• Clone Number: CR3022
• Tested applications: Crystallography, ELISA, IF
• Reactivity: Virus
• Isotype: IgG1 kappa
• Immunogen: The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P59594
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Spike protein, COVID19, COVID 19, S protein, SARS-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (orb1671578) to SARS-CoV-2 Spike Glycoprotein (S1)- Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2)- Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1)- Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2)- Sheep Fc-Tag (orange line) from HEK293 cells (The Native Antigen Company) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using orb1671578. For detection- a 1:4000 dilution of HRP-lnti-human IgG antibody was used.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (orb1671578) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1)- His-Tag (Insect Cells; grey line)- SARS-CoV-2 Spike Glycoprotein (S2)- His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1)- His-Tag (HEK293 cells; blue line) (The Native Antigen Company) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using orb1671578. For detection- a 1:4000 dilution of HRP-lnti-human IgG antibody was used.

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1)- His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2)- His-Tag (Insect Cells; yellow line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using orb1671578; from 370 ng/ml for 6.0 and from 10000 ng/ml for 5.0 and 1.0. Human IgM- human IgA and human IgG2 were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-lnti-human IgG antibody was used.