Covid-19 & SARS-CoV Nucleoprotein Antibody [CR3009 (03-009)], Human IgG1

• Catalog Number: orb1671554
• Category: Antibodies
• Description: Covid-19 & SARS-CoV Nucleoprotein Antibody [CR3009 (03-009)], Human IgG1
• Clonality: Recombinant
• Clone Number: CR3009 (03-009)
• Tested applications: ELISA, IF
• Reactivity: Virus
• Isotype: IgG1 kappa
• Immunogen: The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original antigen was the whole irradiated virion.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: N
• UniProt ID: P0DTC9, P59595
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: NP, NC, Protein N, Nucleocapsid protein, SARS-CoV
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MDCK-SIAT1 cells transfected with SARS-CoV-2 NP with anti-Covid-19 & SARS-CoV Nucleoprotein antibody CR3009 (03-009) (Immunofluorescence analysis of MDCK-SIAT1 cells stsfected with SARS-CoV-2 NP. The cells were seeded in a flat bottomed 96 well plate overnight- fixed in 10% formalin at 4C for 30min- permefor 20min at RT and then stained with the human IgG1 version of CR3009 (03-009) (orb1671554) in PBS/0.1% BSA at 10 ug/ml for 1 hour followed by a goat anti-human Alexa Fluor® 647 (Invitrogen) secondary antibody.