ATP7B Antibody (APC)

SKU: orb149809

Description

Mouse monoclonal to Copper Transporting ATPase 2 (APC). The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver, characterized by neurological symptoms and hepatic damage..

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: L62/29 (Formerly sold as S62-29)
• Immunogen: Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2
• Target: ATP7B
• Molecular Weight: 160kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP7B, ATPase Cu++ transporting beta polypeptide, ATPase Cu(2+) transporting beta polypeptide, Copper pump 2, Copper transporting ATPase 2, PWD, Toxic milk, tx, WC1, WD, Wilson disease associated protein, WND, WND/140 kDa

Quick Database Links

Gene Symbol

ATP7B

UniProt

B7ZLR4

NCBI Gene ID

540

RefSeq (Protein):NP_000044.2