COPE Antibody

• Catalog Number: orb402549
• Category: Antibodies
• Description: COPE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 34482 MW
• UniProt ID: O14579
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Coatomer subunit epsilon;Epsilon-coat protein;Epsi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-COPE antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-COPE antibody.Lane 1:rat stomach Tissue;2:rat small intestine Tissue;3:rat pancreas Tissue;4:mouse stomach Tissue;5:mouse small intestine Tissue;6:mouse pancreas Tissue;7:human MCF-7 Cellm;8:human HeLa Cell;9:human 22RV1 Cell.

IF analysis of COPE using anti-COPE antibody.COPE was detected in immunocytochemical section of U20S cell.

IF analysis of COPE using anti-COPE antibody.COPE was detected in immunocytochemical section of MCF-7 cell.

IHC analysis of COPE using anti-COPE antibody.COPE was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of COPE using anti-COPE antibody.COPE was detected in paraffin-embedded section of mouse pancreas tissue.

IHC analysis of COPE using anti-COPE antibody.COPE was detected in paraffin-embedded section of rat pancreas tissue.