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Catalog Number | orb1522846 |
---|---|
Category | Antibodies |
Description | COPB2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 856 and 906 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | COPB2 |
UniProt ID | P35606 |
NCBI | NP_004757.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | p102; beta'-COP; betaprime-COP; coatomer binding c Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human COPB2 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, HeLa, U2OS, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of mouse COPB2 by western blot. Samples: Whole cell lysate (5 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for IP at 6 µg per reaction. COPB2 was also immunoprecipitated by a previous lot of this antibody (orb1522846) and a second antibody against a different epitope of COPB2.
Detection of human COPB2 by immunohistochemistry. Samples: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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