COPB2 Antibody

• Catalog Number: orb1522846
• Category: Antibodies
• Description: COPB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 856 and 906
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: COPB2
• UniProt ID: P35606
• NCBI: NP_004757.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p102; beta'-COP; betaprime-COP; coatomer binding c
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human COPB2 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, HeLa, U2OS, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of mouse COPB2 by western blot. Samples: Whole cell lysate (5 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody (orb1522846) used for IP at 6 µg per reaction. COPB2 was also immunoprecipitated by a previous lot of this antibody (orb1522846) and a second antibody against a different epitope of COPB2.

Detection of human COPB2 by immunohistochemistry. Samples: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB