C4A, C4B Antibody

SKU: orb1251876

Description

C4A, C4B Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human
• Application Notes:
  ELISA: 1-2 µg/mL (order BSA/azide free format)Immunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM545
• Immunogen: Recombinant human C4d protein was used as the immunogen for this Complement 4d antibody.
• Target: C4A, C4B
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, Complement C4 beta chain, Complement C4-A alpha chain, C4a anaphylatoxin, C4b-A, C4d-A, Complement C4 gamma chain, C4A, CO4, CPAMD2

Formalin-fixed, paraffin-embedded human kidney transplant stained with Complement 4d antibody (SPM545).

Quick Database Links

Gene Symbol

C4A, C4B

UniProt

P0C0L5, P0C0L4