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Item 1 of 4
Item 1 of 4
Collagen XVII Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb500086
| Catalog Number | orb500086 |
|---|---|
| Category | Antibodies |
| Description | Collagen XVII Recombinant Rabbit Monoclonal Antibody |
| Target | COL17A1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/1ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | Recombinant human Collagen XVII protein (1300-1450/1497aa) |
| UniProt ID | Q9UMD9 |
| MW | 150 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 1C11 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Bullous Pemphigoid 180; 180 kDa bullous pemphigoid Read more... |
| Note | For research use only |
25 ug total protein per lane of various lysates probed with Collagen XVII monoclonal antibody, unconjugated (orb500086) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
