Collagen IV/COL4A1 Antibody

• Catalog Number: orb196269
• Category: Antibodies
• Description: Collagen IV/COL4A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 138564 MW
• UniProt ID: P02462
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Collagen alpha-1 (IV) chain; Arresten; COL4A1
• Note: For research use only
• Application notes: WB: The detection limit for Collagen IV is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Collagen IV using anti-Collagen IV antibody.Lane 1:human placenta tissue.

IF analysis of COL4A1 using anti-COL4A1 antibody.COL4A1 was detected in paraffin-embedded section of human lung cancer tissues.

IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody.COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues.

IHC analysis of Collagen using anti-Collagen antibody.Collagen was detected in paraffin-embedded section of human lung cancer tissues.