Collagen IV Antibody / COL4A3

• Catalog Number: orb2636407
• Category: Antibodies
• Description: Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1400-1670) of human COL4A protein was used as the immunogen for the Collagen IV antibody.
• UniProt ID: P29400, P08572, P53420, P02462
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Collagen IV antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: COL4A3/9395R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Collagen IV antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human pancreas tissue with Collagen IV antibody (clone COL4/9395R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free COL4A antibody (clone COL4/9395R) as confirmation of integrity and purity.