Collagen II/COL2A1 Antibody

• Catalog Number: orb137988
• Category: Antibodies
• Description: Collagen II/COL2A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Rat, MouseImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 141973 MW
• UniProt ID: P28481
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Collagen alpha-1 (II) chain;Alpha-1 type II collag
• Note: For research use only
• Application notes: WB: The detection limit for COL2A1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Heart Tissue Lysate, Lane 2: Rat Brain Tissue Lysate using COL2A1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Trachea Tissue using COL2A1 antibody