COL9A1 Antibody

• Catalog Number: orb1264616
• Category: Antibodies
• Description: COL9A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 92 kDa
• Target: COL9A1
• UniProt ID: P20849
• NCBI: P20849
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Collagen alpha-1(IX) chain, COL9A1
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in MCF-7 cell line and mouse eyeball, rat eyeball tissue lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.