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Catalog Number | orb345832 |
---|---|
Category | Antibodies |
Description | CO1A2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, IP, WB |
Reactivity | Bovine, Human |
Isotype | IgG |
Immunogen | Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:10,000 - 1:50,000, FC: User Optimized, IHC: 1:200 - 1:1,000, IF: User Optimized, IP: 1:100, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
Conjugation | FITC |
UniProt ID | P02458 |
NCBI | NP_001835.3 |
Storage | Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Collagen Type II antibody fluorescein Read more... |
Note | For research use only |
Application notes | Anti-Collagen Type II Fluorescein Conjugated Antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit |
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