CNTF Antibody

• Catalog Number: orb180710
• Category: Antibodies
• Description: CNTF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CNTF recombinant protein (Position: A2-M200). Human CNTF shares 83% and 84% amino acid (aa) sequences identity with mouse and rat CNTF, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22931 MW
• UniProt ID: P26441
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ciliary neurotrophic factor;CNTF;CNTF;
• Note: For research use only
• Application notes: WB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CNTF using anti-CNTF antibody.Lane 1:Recombinant Human CNTF Protein 0.5ng.

IHC analysis of CNTF using anti-CNTF antibody. CNTF was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CNTF using anti-CNTF antibody. CNTF was detected in paraffin-embedded section of human lung cancer tissues.