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Catalog Number | orb1247710 |
---|---|
Category | Antibodies |
Description | CNR1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine, Human, Mouse |
Reactivity | Rat |
Immunogen | The immunogen for this antibody is: C-HTSEDGKVQVTRPDQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Rat Brain lysates (calculated MW of 52.7kDa according to Rat NP_036916.1 and 52.9kDa according to Human NP_057167.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Human: Fügedi G et al, BMC Pregnancy Childbirth. 2014 Dec 2;14:395. PMID: 25444073. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CNR1 |
UniProt ID | P21554 |
NCBI | NP_001153730.1, NP_149421.1, NP_057167.2, NP_001153698.1, NP_001153732.1, NP_001153731.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | cannabinoid receptor 1 (brain), CNR1, HGNC:2159, C Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Rat Brain lysates (calculated MW of 52.7kDa according to Rat NP_036916.1 and 52.9kDa according to Human NP_057167.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Human: Fügedi G et al, BMC Pregnancy Childbirth. 2014 Dec 2;14:395. PMID: 25444073. |
Expiration Date | 12 months from date of receipt. |
orb1247710 (0.1 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
ELISA, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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