CNOT2 Antibody

• Catalog Number: orb1526410
• Category: Antibodies
• Description: CNOT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 490 and 540
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. orb1526410 has not performed satisfactorily when used for WB of CNOT2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CNOT2.; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CNOT2
• UniProt ID: Q9NZN8
• NCBI: NP_055330.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CCR4-NOT transcription complex subunit 2; NOT2H; N
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CNOT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CNOT2 antibody orb1526410 used for IP at 10 µg/mg lysate.

Detection of human CNOT2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CNOT (Cat. No. orb1526410) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB