CNN3 Antibody

• Catalog Number: orb1263782
• Category: Antibodies
• Description: CNN3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This Calponin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human Calponin-3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: CNN3
• UniProt ID: Q15417
• NCBI: Q15417
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Calponin-3, Calponin, acidic isoform, CNN3
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Calponin-3 antibody in NIH-3T3 cell line lysates (35 ug/lane)

Calponin-3 Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.