【科研专题】白细胞分化抗原 / CD分子系列

 

CD分化簇（Cluster of Differentiation）抗原，也被称为白细胞分化抗原，是指不同谱系白细胞在正常分化，成熟，活化过程中出现或消失的细胞表面标志，多是跨膜蛋白或糖蛋白，少数为碳水化合物半抗原。这些抗原参与机体的生理和病理过程，并在疾病的诊断、治疗和预后评估中发挥重要作用。

这一期，biorbyt 就来和大家聊聊白细胞分化抗原 / CD分子!

 

 

CD分子的主要功能

白细胞分化抗原(Leucocyte Differentiation Antigen)参与机体重要的生理和病理过程，主要包括以下主要功能：   

① 免疫应答过程中免疫细胞的相互识别，免疫细胞抗原识别、活化、增殖和分化，免疫效应功能的发挥；   

② 白细胞分化抗原在造血细胞的分化和造血过程中起着关键的调控作用；   

③ 白细胞分化抗原也参与炎症反应、血栓形成和组织修复等生理和病理过程。   

④ 这些抗原还参与细胞的生长、分化、迁移过程，并在肿瘤的恶化和转移中发挥作用。

 

 

CD分子的命名与分类

目前，人类CD抗原编号现已达到CD363，已有70余种CD抗原被鉴定。可大致划分为T细胞、B细胞、髓细胞 、NK（自然杀伤）细胞等。不同序号的CD可有同一组细胞，如CD3/CD7同为T细胞，CD22/CD37同为B细胞；同时同一序号CD可有几种细胞组，如CD5为T细胞，B细胞亚群。   

 

 

常见CD抗原和功能

CD系列抗原不仅参与机体的生理过程，还在疾病的诊断、治疗和预后评估中发挥作用。下面我们对常见的部分CD抗原进行一一介绍。

➤ CD3

CD3是T细胞受体（TCR）复合体的一部分，对于T细胞的激活和信号传导至关重要。   

Biorbyt 可提供高特异性，经过严格质检的CD3抗体，部分热销货号质检结果如下。

产品质检结果

1）Biorbyt，orb1175，CD3 antibody，WB质检结果图

 

2）Biorbyt，orb348965，CD3 antibody，IHC质检结果图

 

3）Biorbyt orb10313 CD3 antibody，IF质检结果

orb10313发表文献：

1. Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021) 

2. Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021) 

 

➤ CD4

CD4是辅助T细胞的主要表面标志，这些T细胞调控其他淋巴细胞的功能。CD4的降低可能出现在恶性肿瘤、遗传性免疫缺陷症、艾滋病以及应用免疫抑制剂的情况下。   

Biorbyt 可提供高特异性，经过严格质检的CD4抗体，部分热销货号质检结果如下。

产品质检结果

1）Biorbyt，orb4830，CD4 antibody，WB质检结果图

2) Biorbyt，orb4830，CD4 antibody，IHC质检结果图 

 

3) Biorbyt，orb4830，CD4 antibody，IF质检结果图 

orb4830发表文献：

1. Su, Jiyan et al. Protective Effect of Pogostone on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis via Inhibition of T Helper Cell Front Pharmacol, 8, 829 (2017)  

2. Romero-Moreno, Ricardo et al. The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis Nat Commun, 10, 4404 (2019)

 

➤ CD8

CD8是细胞毒T细胞（也称为杀手T细胞）的主要表面标志，负责杀灭产生特殊抗原反应的目标细胞，如受感染的细胞。CD8的减低可能与自身免疫性疾病或变态反应性疾病有关。   

Biorbyt 可提供高特异性，经过严格质检的CD8抗体，部分货号质检结果如下。

产品质检结果

1）Biorbyt，orb312181，CD8 antibody，IF 质检结果图: 

 

2）Biorbyt，orb10325，CD8 antibody，WB质检结果图: 

orb10325发表文献：   

1. Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021) 

2. Chien-Chun Steven Pai et al. Clonal Deletion of Tumor-Specific T Cells by Interferon-? Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade Immunity, 50, 477-492.e8 (2019) 

3. Chen, Xinran et al. Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice Oncol Lett, 13, 2207-2215 (2017) 

4. Prashant, Chandravilas Keshvan et al. Fabrication of nanoadjuvant with poly-ε-caprolactone (PCL) for developing a single-shot vaccine providing prolonged immunity Int J Nanomedicine, 9, 937-950 (2014) 

5. Pedro Henrique Felix Silva et al. Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023) 

6. Hekim, Can et al. Dasatinib Changes Immune Cell Profiles Concomitant with Reduced Tumor Growth in Several Murine Solid Tumor Models Cancer Immunol Res, 5, 157-169 (2017) 

7. Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021) 

8. Annen, Ryohei et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model Int J Mol Sci, 23, 9445 (2022) 

9. Ka, Na-Lee et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Rep, 37, 110138 (2021)

 

➤ CD19

CD19主要表达在B细胞、B细胞前体和滤泡树突状细胞的表面。参与B细胞的活化、增殖和分化，以及抗体产生。   

Biorbyt 可提供高特异性，经过严格质检的CD19抗体，部分货号质检结果如下。

产品质检结果

1）Biorbyt，orb10306，CD19 antibody，IF 质检结果图: 

2）Biorbyt，orb1699，CD19 antibody，FC质检结果图: 

orb1699 发表文献：

Hao, Wen et al. Aspirin inhibits growth and enhances cardiomyocyte differentiation of bone marrow mesenchymal stem cells Eur J Pharmacol, 827, 198-207 (2018)

 

➤ CD45

CD45也称为白细胞共同抗原，几乎在所有白细胞表面表达，是蛋白酪氨酸磷酸酶的跨膜受体。参与细胞信号传导和细胞间相互作用，对免疫应答的调节起到重要作用。   

Biorbyt 可提供高特异性，经过严格质检的CD45抗体，部分货号质检结果如下。

产品质检结果

1）Biorbyt，orb688878，CD45 antibody，WB 质检结果图:

2）Biorbyt，orb688878，CD45 antibody，FC 质检结果图: 

 

3）Biorbyt，orb688878，CD45 antibody，IF 质检结果图:

 

➤ CD56

CD56主要表达在自然杀伤（NK）细胞和某些T细胞亚群的表面。参与NK细胞的活化和功能，包括细胞毒性作用和细胞因子产生。在某些肿瘤免疫治疗中，NK细胞表面的CD56可能成为治疗靶点。   

Biorbyt 可提供高特异性，经过严格质检的CD56抗体，部分货号质检结果如下。

产品质检结果

1）Biorbyt，orb373851，CD56 antibody，WB质检结果图: 

2）Biorbyt，orb373851，CD56 antibody，IF质检结果图: 

orb373851发表文献：

Qin, Chen-Jie et al. Inhibition of dipeptidyl peptidase IV prevents high fat diet-induced liver cancer angiogenesis by downregulating chemokine ligand 2 Cancer Lett, 420, 26-37 (2018) 
 

➤ CD133

CD133也称为PROM1（Prominin-1），是造血干细胞表面的糖蛋白，是调控干细胞命运的关键分子，是干细胞的功能性标志物。在HSCs（造血干细胞）分化成熟过程中，CD133的含量迅速降低。CD133在干细胞研究、肿瘤研究和生物技术应用中扮演着重要角色，是一个多功能的细胞表面标志。   

Biorbyt 可提供高特异性，经过严格质检的CD133抗体，部分货号质检结果如下。

1）Biorbyt，orb99113，CD133 antibody，WB 质检结果图:

2）Biorbyt，orb99113，CD133 antibody，IHC 质检结果图:

3）Biorbyt，orb99113，CD133 antibody，IF质检结果图:

orb99113发表文献：   

1. Feng, Long et al. Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer Mol Med Rep, 12, 3531-3536 (2015) 

2. Winardi, Daniel et al. Correlation of altered expression of the autophagy marker LC3B with poor prognosis in astrocytoma Biomed Res Int, 2014, 723176 (2014) 

3. Chen, Jintao et al. Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma J Histochem Cytochem, (2021) 

4. Feitosa, Neli Patrícia Pereira et al. Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133 Acta Histochem, 123, 151787 (2021) 

5. Kase, Marju et al. Impact of CD133 positive stem cell proportion on survival in patients with glioblastoma multiforme Radiol Oncol, 47, 405-410 (2013) 

6. Hirashima, Kanji et al. Cell biological profiling of reprogrammed cancer stem cell-like colon cancer cells maintained in culture Cell Tissue Res, 375, 697-707 (2019) 

7. Nowacki, Maciej et al. Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis Oncotarget, 6, 22776-22798 (2015) 

8. Zou, Yuhong et al. Nrf2 is involved in maintaining hepatocyte identity during liver regeneration PLoS One, 9, e107423 (2014) 

9.Coni, Pierpaolo et al. Exploring cell surface markers and cell-cell interactions of human breast milk stem cells J Public Health Res, 12, (2023) 

 

 

CD分子的临床应用

CD检测在细胞生物学研究、疾病诊断、疗效监测与预后评估以及免疫研究和药物研发等方面有广泛应用。   

例如，在白血病、淋巴瘤等恶性疾病的诊断中，CD检测可以辅助医生确定诊断，提高准确率，并指导后续的治疗方案。   

总之，CD系列抗原是白细胞表面的一类重要抗原标志，对于理解人体的免疫反应和疾病的发生发展具有重要意义。通过检测这些抗原的表达情况，可以为疾病的诊断、治疗和预后评估提供重要信息。

除了以上列举的常用CD分子抗体，Biorbyt 还可提供更多高特异性的CD系列的抗体，部分热销货号如下，欢迎咨询。

Target	Catalog No.	Application	Reactivity
CD10	orb10284	WB,IHC,IF,FC	Human, Mouse, Rat
CD197	orb10276	WB,IHC,IF,FC	Human, Mouse, Rat, Canine
CD195	orb13298	WB,IHC,IF,FC	Human, Mouse, Rat
CD105	orb101826	IF, WB	Human, Mouse, Rat
CD11b	orb11009	WB,IHC,IF,FC	Human,Mouse, Rat, Porcine
CD11c	orb500955	FC, IF	Human, Rat, Canine, Mouse
CD137	orb13300	WB	Human, Mouse
CD138	orb10289	IF, IHC, WB	Bovine, Human, Mouse, Rat, Canine,Equine,Gallus,Porcine, Rabbit
CD14	orb499573	IF,IHC,WB	Human,Mouse,Bovine,Canine,Porcine,Rabbit, Rat, Sheep
CD142	orb317979	IF, IHC, WB	Human, Mouse, Rat, Bovine, Canine,Equine,Guinea pig, Porcine, Rabbit
CD146	orb500881	WB	Human,Rat,Bovine,Canine, Equine, Mouse
CD147	orb10297	WB,IHC,IF,FC	Human, Mouse, Rat, Bovine, Canine,Gallus,Guinea pig, Porcine, Rabbit
CD151	orb13358	WB,IF	Human,Mouse,Bovine,Equine,Gallus,Rat
CD158i	orb5814	IF,  IHC,  WB	Human
CD160	orb13302	FC	Human,Canine,Mouse,Rat
CD163	orb182468	WB,IHC,IF,FC	Human,Mouse,Canine,Equine,Porcine,Rat
CD166	orb10298	WB,IF,FC	Human,Bovine,Canine,Equine,Gallus,Mouse,Porcine,Rabbit, Rat
CD16	orb101668	WB,IHC,IF,FC	Human, Mouse, Rat, Bovine, Porcine, Rabbit, Sheep
CD177	orb10302	WB,IHC-P	Mouse,Rat
CD19	orb317567	IHC,IF,FC	Human, Mouse, Rat, Bovine, Equine, Guinea pig, Porcine
CD20	orb317568	WB,FC	Human, Mouse, Rat, Bovine, Canine, Rabbit
CD244	orb13331	WB,IF	Human, Rat, Mouse
CD26	orb13310	FC,  IHC-P	Human,Rat,Bovine,Equine, Mouse, Porcine, Rabbit
CD30	orb13314	WB,IHC,IF,FC	Human, Porcine
CD31	orb10315	IHC-P, WB，IF	Bovine,Human,Mouse,Rabbit,Rat,Canine, Porcine, Sheep
CD33	orb10316	WB,IHC,IF	Human, Mouse, Rat
CD34	orb500821	WB,IHC,IF,FC	Rat,Mouse,Human, Dog
CD4	orb312176	IF,  IHC,  WB	Human, Mouse, Rat
CD44	orb13319	FC	Human,Mouse,Bovine,Gallus,Porcine,Rabbit,Rat
CD45	orb1179	WB,IHC,IF,FC	Human, Mouse, Rat
CD47	orb4838	WB,IHC,IF,FC	Human, Mouse, Rat, Rabbit
CD56	orb10338	WB,IHC,IF	Human,Rat,Bovine,Canine,Equine,Gallus,Mouse,Porcine, Rabbit
CD62p	orb500998	WB,IHC,IF,FC	Human,Porcine,Rat,Bovine,Canine, Equine, Mouse
CD64	orb6816	WB,IHC-P,ICC	Human, Mouse, Rat
CD69	orb500673	WB,IHC,IF,FC	Human, Mouse, Canine, Rat
CD73	orb2931	WB,IF,FC	Human,Mouse, Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat, Sheep
CD8	orb312181	WB,IHC,IF	Human, Mouse
CD80	orb5805	WB,IHC,IF	Human, Mouse, Rat
CD86	orb500820	WB,IHC,IF	Human, Mouse, Rat, Bovine, Canine, Porcine, Sheep
CD95	orb10642	WB,IHC,IF	Rat,Mouse,Human
CD98	orb101904	IHC,IF	Guinea pig, Human, Mouse, Porcine, Rabbit, Rat

   
 

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