CLU Antibody

• Catalog Number: orb1672042
• Category: Antibodies
• Description: CLU Antibody
• Clonality: Recombinant
• Clone Number: SAIC-43B-8
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "ASSIIDELFQDR" derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CLU
• UniProt ID: P10909
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Clusterin, APOJ, CLI, KUB1, AAG4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CLU antibody SAIC-43B-8. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-43B-8 (orb1672042 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-CLU antibody SAIC-43B-8. HeLa(A) (0.03 ug/ml)- MCF7(B) (0.01 ug/ml) cell lysate and human brain cerebellum(C) (0.03 ug/ml)- human plasma (D) (0.03 ug/ml) and human testes(E) (0.03 ug/ml) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-43B-8 (orb1672042) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of HeLa cells with anti-CLU (SAIC-43B-8). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-43B-8 (orb1672042) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672042- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.