CLU Antibody

• Catalog Number: orb1247667
• Category: Antibodies
• Description: CLU Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EKALQEYRKKHREE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CLU
• UniProt ID: P10909
• NCBI: NP_001822.2, NP_976084.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CLU, APOJ, clusterin, complement lysis inhibitor,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
• Expiration Date: 12 months from date of receipt.

orb1247667 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247667 (0.1 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247667 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing ER/cytoplasmic staining. The nuclear stain is DAPI.