CLTB antibody

• Catalog Number: orb412712
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CLTB
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Clathrin Light Chain B
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CLTB
• Entrez: 1212, 116561, 74325
• UniProt ID: P09497, Q6IRU5, P08082
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-Clathrin light chain B antibody, anti-Lcb ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Clathrin Light Chain B expression in A549 (A), MCF7 (B), NIH3T3 (C), mouse lung (D) whole cell lysates. (Predicted band size: 23; 25 kD; Observed band size: 30 kD)

Immunofluorescent analysis of Clathrin Light Chain B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).