CLTA Antibody

• Catalog Number: orb1246648
• Category: Antibodies
• Description: CLTA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CDFNPKSSKQAKD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CLTA
• UniProt ID: P09496
• NCBI: NP_001070145.1, NP_009027.1, NP_001825.1, NP_001824.1, NP_009028.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CLTA, CTLB, clathrin, light chain (Lca), LCA, clat
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246648 (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246648 (2.5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.