CLPS Antibody / Colipase

• Catalog Number: orb2636381
• Category: Antibodies
• Description: The lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Members of the AB hydrolase subfamily include Hepatic Lipase (HL), Endothelial Lipase (EL), Lipoprotein Lipase (LPL), Pancreatic Lipase (PL), Gastric Lipase (GL) and LCAT. These family members play a crucial role in the metabolism of lipids. Pancreatic lipase, also designated pancreatic triacylglycerol acyl hydrolase, is important for dietary fat absorption as it hydrolyses triglycerides into diglycerides, monoglycerides and free fatty acids. Colipase, also known as CLPS or pancreatic colipase preproprotein, is a 112 amino acid secreted protein that functions as a cofactor of pancreatic lipase. Necessary for dietary lipid hydrolysis and localizing to pancreatic acinar cells, colipase allows pancreatic lipase to anchor itself to the lipid-water interface of lipid micelles, thereby preventing intestinal bile salts from washing it off.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLPS/9026
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant fragment (within amino acids 1-112) of human Colipase protein was used as the immunogen for the Colipase antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CLPS antibody is available for research use only.
• UniProt ID: P04118
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Colipase antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human pancreas tissue lysate with Colipase antibody (clone CLPS/9026). Predicted molecular weight ~12 kDa.

IHC staining of FFPE human pancreas tissue with Colipase antibody (clone CLPS/9026). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Colipase antibody (clone CLPS/9026) as confirmation of integrity and purity.