CLIP1 antibody

• Catalog Number: orb20278
• Category: Antibodies
• Description: Goat polyclonal antibody to CLIP1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 161; 157
• Target: Restin / CLIP1
• Entrez: 6249
• Protein Sequence: PHSTHHGSRGEERP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CLIP1 antibody, anti CAP-GLY domain containin
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows structured cytoplasm staining in active epithelial cells. Recommended concentration, 2-4ug/ml.WB: Approx 160kDa band observed in Human Breast Cancer lysates (calculated MW of 161kDa according to NP_002947.1. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20278 (1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20278 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Prostate using CLIP1 antibody.