CLIC4 antibody

• Catalog Number: orb19557
• Category: Antibodies
• Description: Goat polyclonal antibody to CLIC4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 28.8
• Target: CLIC4
• Entrez: 25932
• Protein Sequence: NGLKEEDKEPLIE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CLIC4 antibody, anti chloride intracellular c
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar;276(3 Pt 2):F398-408.; PMID: 10070163). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5ug/ml with overnight incubation.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human kidney lysate (0.1ug/ml) using CLIC4 antibody

Confocal immunofluoroscence analysis of min6 cells using CLIC4 antibody

Immunohistochemical staining of Human Heart using CLIC4 antibody