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Catalog Number | orb2291791 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant CLIC3. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3F8 |
Tested applications | ELISA, IF, IHC-P, IP, WB |
Reactivity | Human |
Isotype | IgG2a Kappa |
Immunogen | CLIC3 (NP_004660, 137 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | RLDSYLRAPLEHELAGEPQLRESRRRFLDGDRLTLADCSLLPKLHIVDTVCAHFRQAPIPAELRGVRRYLDSAMQEKEFKYTCPHSAEILAAYRPAVHPR |
NCBI | NP_004660 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
CLIC3 monoclonal antibody (M02), clone 3F8 Western Blot analysis of CLIC3 expression in A-431.
Detection limit for recombinant GST tagged CLIC3 is approximately 0.1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to CLIC3 on HeLa cell. [antibody concentration 15 ug/ml]
Immunoperoxidase of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoprecipitation of CLIC3 transfected lysate using anti-CLIC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLIC3 MaxPab rabbit polyclonal antibody.
Western Blot analysis of CLIC3 expression in transfected 293T cell line by CLIC3 monoclonal antibody (M02), clone 3F8. Lane 1: CLIC3 transfected lysate (26.6 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa).