CLEC9A Antibody

• Catalog Number: orb2644097
• Category: Antibodies
• Description: This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 1F6
• Tested applications: FACS, IF, WB
• Reactivity: Mouse
• Isotype: Rat IgG1, kappa
• Immunogen: RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CLEC9A antibody is available for research use only.
• UniProt ID: Q8RU4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 1F6) as confirmation of integrity and purity.