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Catalog Number | orb2643319 |
---|---|
Category | Antibodies |
Description | This MAb recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 8F9 |
Tested applications | FACS, IF |
Reactivity | Human |
Isotype | Mouse IgG2a, kappa |
Immunogen | RBL-2H3 cells expressing human CLEC9A fused to an HA epitope. Its epitope maps to within amino acids 50-110. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This CLEC9A antibody is available for research use only. |
UniProt ID | Q8RU4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 8F9) as confirmation of integrity and purity.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |