CLDN4 Antibody

• Catalog Number: orb1239033
• Category: Antibodies
• Description: CLDN4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-CLAUDIN4 antibody (orb1239033) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CLAUDIN4. The immunogen is located within the last 50 amino acids of CLAUDIN4.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 22kDObserved: 22 kD
• Target: CLDN4
• UniProt ID: O14493
• NCBI: NP_001296
• Storage: CLAUDIN4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CLAUDIN4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CLAUDIN4 Antibody: CPER, CPE-R, CPETR, CPETR1, WBS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (1 µg/mL), CLAUDIN4 (1 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.