CLDN1 monoclonal antibody (M02), clone 2E2-H5

• Catalog Number: orb2291775
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant CLDN1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2E2-H5
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG2b Kappa
• Immunogen: CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
• NCBI: AAH12471.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged CLDN1 is 0.3 ng/ml as a capture antibody.

Huh7 cells were stained with CLDN1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

Immunofluorescence of monoclonal antibody to CLDN1 on Huh7 cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.

Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M02), clone 2E2-H5. Lane 1: CLDN1 transfected lysate (Predicted MW: 22.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (48.95 KDa).