CLCN2 monoclonal antibody (M01), clone 3E1

• Catalog Number: orb2294366
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant CLCN2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: CLCN2 (AAH21578.1, 38 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
• NCBI: AAH21578.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in Hela S3 NE.

Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.

Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1. Lane 1: CLCN2 transfected lysate(96.8 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # orb2294366). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (64.24 KDa).