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Catalog Number | orb1953196 |
---|---|
Category | Proteins |
Description | Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484. |
Tag | C-His-Avi |
Purity | 98.00% |
MW | 22.44 kDa (predicted) |
UniProt ID | O95484 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484. |
Expression Region | A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. Predicted N terminal: Met 1 |
Storage | -20°C |
Note | For research use only |
Application notes | 0 |
Expiration Date | 6 months from date of receipt. |