Claudin 2/CLDN2 Antibody

• Catalog Number: orb234269
• Category: Antibodies
• Description: Claudin 2/CLDN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Rat, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 24549 MW
• UniProt ID: P57739
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Claudin-2;SP82;CLDN2;PSEC0059, SP82, UNQ705/PRO135
• Note: For research use only
• Application notes: WB: The detection limit for Claudin 2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Rat Kidney Tissue Lysate(lane 1), Rat Liver Tissue Lysate(lane 2) using CLDN2 antibody.

Immunohistochemical staining of Human Intestinal Cancer Tissue using CLDN2 antibody.