CKM Antibody

• Catalog Number: orb1263492
• Category: Antibodies
• Description: CKM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rabbit
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: CKM
• UniProt ID: P06732
• NCBI: P06732
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Creatine kinase M-type, Creatine kinase M chain, M
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Antibody is used in Western blot to detect CKM in C6 cell lysate.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.