CKB Antibody / Creatine kinase B / Creatine Phosphokinase-BB

• Catalog Number: orb2636234
• Category: Antibodies
• Description: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CKBB/8306R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human full-length Creatine kinase B protein was used as the immunogen for the recombinant Creatine kinase B antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Creatine kinase B antibody is available for research use only.
• UniProt ID: P12277
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Creatine kinase B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain tissue with recombinant Creatine kinase B antibody (clone CKBB/8306R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HEK293T cell lysate using recombinant Creatine kinase B antibody (clone CKBB/8306). Predicted molecular weight ~43 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Creatine kinase B antibody (clone CKBB/8306R) as confirmation of integrity and purity.