CKB Antibody / Creatine kinase B

• Catalog Number: orb1716150
• Category: Antibodies
• Description: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CKBB/6565
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human full-length protein was used as the immunogen for the CKB antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: for coating, order antibody without BSA,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CKB antibody is available for research use only.
• UniProt ID: P12277
• Storage: Aliquot the CKB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the CKB antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with CKB antibody (clone CKBB/6565). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with CKB antibody (clone CKBB/6565). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Y79 cell lysate using CKB antibody (clone CKBB/6565). Predicted molecular weight ~43 kDa.

Western blot testing of human HEK293 cell lysate using CKB antibody (clone CKBB/6565). Predicted molecular weight ~43 kDa.