CK5 Antibody

SKU: orb333942

Description

Mouse monoclonal antibody to CK5

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 2C8E4
• Immunogen: Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.
• Target: CK5
• Molecular Weight: 62.4kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

KRT5; K5; DDD; DDD1; EBS2; KRT5A

Immunofluorescence analysis of MCF-7 cells using CK5 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded lung cancer tissues using CK5 antibody

Western blot analysis of human recombinant protein using CK5 antibody

Western blot analysis of HEK293 using CK5 antibody

Western blot analysis of A431 (Lane1), MCF-7 (Lane2), SK-Br-3 (Lane3), Hela (Lane4), Lncap (Lane5), and HepG2 (Lane6) cell lysate using CK5 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using CK5 antibody

Quick Database Links

Gene Symbol

CK5

UniProt

P13647

NCBI Gene ID

3852