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Catalog Number | orb333942 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CK5 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2C8E4 |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli. |
Antibody Type | Primary Antibody |
Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 62.4kDa |
Target | CK5 |
Entrez | 3852 |
UniProt ID | P13647 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Alternative names | KRT5; K5; DDD; DDD1; EBS2; KRT5A Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of MCF-7 cells using CK5 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded lung cancer tissues using CK5 antibody
Western blot analysis of human recombinant protein using CK5 antibody
Western blot analysis of HEK293 using CK5 antibody
Western blot analysis of A431 (Lane1), MCF-7 (Lane2), SK-Br-3 (Lane3), Hela (Lane4), Lncap (Lane5), and HepG2 (Lane6) cell lysate using CK5 antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of Hela cells using CK5 antibody
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Mouse | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |