CK18 Mouse Monoclonal Antibody

SKU: orb499583

Description

CK18 Mouse Monoclonal Antibody

Research Area

Cancer Research, Signal Transduction, Tumor Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 3C3
• Immunogen: Recombinant human CK18
• Target: KRT18
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (orb499583) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: A431 Cell (Human) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Sample: Lane 1: Small intestine (Rat) Lysate at 40 ug, Lane 2: Pancreas (Rat) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Lane 4: Uterus (Rat) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 45 kD.

Sample: MCF-7 Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

KRT18

UniProt

P05783