CIP2A Antibody

• Catalog Number: orb1520147
• Category: Antibodies
• Description: CIP2A Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Amino terminal one-third of the CIP2A (GeneID 57650) recombinant protein
• Concentration: 40 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CIP2A
• UniProt ID: Q8TCG1
• NCBI: NP_065941.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: KIAA1524; protein CIP2A; p90; cancerous inhibitor
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse CIP2A by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, CT26, Ba/F3, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520147) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CIP2A by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Jurkat, U2OS, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520147) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human CIP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520147) used for IP at 20 µl/mg lysate.