Chrysophanol

SKU: orb1304759

Description

Chrysophanol is a natural anthraquinone found in plants like rhubarb and sorrel, functioning as a dual inhibitor of the EGFR and mTOR signaling pathways. It is utilized in biochemical research for investigating cell proliferation and apoptosis, with studies demonstrating its activity in both in vitro and in vivo cancer models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 481-74-3
• MW: 254.24
• Purity: 99.70%
• Formula: C₁₅H₁₀O₄
• SMILES: Cc1cc(O)c2C(=O)c3c(O)cccc3C(=O)c2c1
• Target: EGFR
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:1.64 mg/mL (6.45 mM)

Bioactivity

• In Vivo:
  Chrysophanol (CA) has been shown to counteract obesity in C57BL/6 mice induced by a high-fat diet (HFD). Through in vivo studies involving male C57BL/6J mice, it was discovered that Chrysophanol significantly mitigates weight gain associated with HFD consumption. Specifically, mice subsisting on an HFD without Chrysophanol treatment experienced a weight increase of 23.92 ± 1.74 g over 16 weeks. In contrast, mice treated with Chrysophanol exhibited a markedly lower weight gain of 16.72 ± 2 g in the same period, underscoring Chrysophanol's potential as an effective intervention against HFD-induced obesity.
• Cell Research:
  Chrysophanol (Chrysophanic Acid) is dissolved in DMSO and stored, and then diluted with appropriate medium before use. The cells are seeded at 5×103 cells/mL in 96-well microplates and allowed to attach for 24 h. Chrysophanol (20, 50, 80 and 120 μM) is added to the medium at various concentrations up to 120 μM and for different durations. After treatment, cell cytotoxicity and/or proliferation is assessed by a Cell Counting Kit-8 (CCK-8). Briefly, highly water-soluble tetrazolium salt, WST-8, produces an orange colored water-soluble product, formazan. The amount of formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.CCK-8 (10 μL) is added to each well and incubated for 3 h at 37°C, then cell proliferation and cytotoxicity are assessed by measuring the absorbance at 450 nm using a microplate reader.Three replicated wells are used for each experimental condition.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chrysophanic Acid, Chrysophanol, 3-Methylchrysazin, inhibit, EGFR, ErbB-1, Epidermal growth factor receptor, Inhibitor, HER1, Turkey Rhubarb

Compound Identifiers

PubChem CID

10208