CHRM3 / M3 Antibody (Cytoplasmic Domain)

• Catalog Number: orb1533496
• Category: Antibodies
• Description: CHRM3 / M3 Antibody (Cytoplasmic Domain)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P
• Reactivity: Bat, Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum (100%); Guinea pig, Turkey, Chicken, Platypus, Lizard (94%).
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml)
• Conjugation: Unconjugated
• Target: CHRM3 / M3
• Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
• Alternative names: CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 r
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.