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Catalog Number | orb344235 |
---|---|
Category | Antibodies |
Description | Choline Oxidase antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | Choline Oxidase [Alcaligenes species] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000 |
Form/Appearance | Lyophilized |
Purity | Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown. |
Conjugation | HRP |
UniProt ID | P16101 |
NCBI | 116367 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | goat anti-Choline Oxidase Antibody HRP Conjugation Read more... |
Note | For research use only |
Application notes | Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of used to detect Choline Oxidase. Samples of ~1 and 0.25 ug of purified Choline Oxidase per lane using Choline Oxidase antibody (Peroxidase)
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