Choline Acetyltransferase/CHAT Antibody

• Catalog Number: orb18695
• Category: Antibodies
• Description: Choline Acetyltransferase/CHAT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 82536 MW
• UniProt ID: P28329
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Choline O-acetyltransferase;CHOACTase;ChAT;Choline
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CHAT using anti-CHAT antibody.Lane 1:rat testis tissue;2:mouse testis tissue.

IHC analysis of CHAT using anti-CHAT antibody.CHAT was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of CHAT using anti-CHAT antibody.CHAT was detected in paraffin-embedded section of rat brain tissues.