CHMP2B Antibody

• Catalog Number: orb1522884
• Category: Antibodies
• Description: CHMP2B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 163 and 213
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CHMP2B
• UniProt ID: Q9UQN3
• NCBI: NP_054762.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Chromatin-modifying protein 2b; VPS2-2; VPS2 homol
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse CHMP2B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 and (20 µg) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHMP2B antibody orb1522884 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CHMP2B by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHMP2B (Cat. No. orb1522884) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).