CHK2 (phospho-T383) antibody

• Catalog Number: orb235096
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CHK2 (phospho-T383)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T383 of human CHK2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CHEK2
• Entrez: 50883, 11200
• UniProt ID: Q9Z265, O96017
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CDS1 antibody, anti CHK2 antibody, anti RAD53
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CHK2 (Phospho-T383) expression in HEK293T (A), HEK293T-UV-30 min (B) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 62 kD)

Immunofluorescent analysis of CHK2 (Phospho-T383) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).