Chk1, Phospho (S317) Antibody

• Catalog Number: orb1522548
• Category: Antibodies
• Description: Chk1, Phospho (S317) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: surrounding serine 317
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Chk1, Phospho (S317)
• UniProt ID: O14757
• NCBI: NP_001265.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: cell cycle checkpoint kinase; serine/threonine-pro
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Chk1, Phospho (S317) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours) , etoposide (100uM for 4 hours) , or untreated. Antibodies: Affinity purified rabbit anti-Chk1, Phospho (S317) antibody orb1522548 used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Chk1, Phospho (S317) by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Chk1, Phospho (S317) (Cat. No. orb1522548) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).