CHIT1 antibody

• Catalog Number: orb377995
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CHIT1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: Recombinant full length protein of human Chitotriosidase
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CHIT1
• Entrez: 1118
• UniProt ID: Q13231
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Chitotriosidase-1; Chitinase-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sklepkiewicz, Piotr et al. Inhibition of CHIT1 as a novel therapeutic approach in idiopathic pulmonary fibrosis Eur J Pharmacol, 919, 174792 (2022)

PubMed: 35122869

B, Dymek et al. Pharmacological Inhibition of Chitotriosidase (CHIT1) as a Novel Therapeutic Approach for Sarcoidosis Journal of inflammation research, 15, (2022)

PubMed: 36199746

Western blot analysis of Chitotriosidase expression in U251 (A), HEK293T (B), mouse spleen (C) whole cell lysates. (Predicted band size: 43; 48; 49; 51 kD; Observed band size: 55 kD)

Immunohistochemical analysis of Chitotriosidase staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Chitotriosidase staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).