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Catalog Number | orb1946459 |
---|---|
Category | Assays and Kits |
Description | Chicken sIgA(Secretory Immunoglobulin A) ELISA Kit |
Reactivity | Gallus |
Range | 781.25-50000 pg/mL |
Target | sIgA |
Sensitivity | 248.5 pg/mL |
Alternative names | S-IgA Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretory Immunoglobulin A(sIgA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretory Immunoglobulin A(sIgA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretory Immunoglobulin A(sIgA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Gallus | |
31.25-2000pg/ml | |
18.75pg/ml |
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