Chicken IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb346929
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Chicken IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, anti-Alkaline Phosphatase, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Donkey anti-Chicken IgG Antibody Alkaline Phosphat
• Note: For research use only
• Application notes: Anti-Chicken IgG Alkaline Phosphatase antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.