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Catalog Number | orb346914 |
---|---|
Category | Antibodies |
Description | Chicken IgG F(c) antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Gallus |
Isotype | IgG |
Immunogen | Chicken IgG F(c) fragment |
Concentration | 1.5 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum. No reaction was observed against Chicken IgG F(ab) . |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-Chicken IgG F(c) Antibody Peroxidase C Read more... |
Note | For research use only |
Application notes | Anti-Chicken IgG F(c) Antibody has been tested in ELISA and Western Blot. Anti-Chicken IgG F(c) Antibody has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product. It is also suitable in IHC. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE | |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain. | |
Chicken |
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